Proteomics Mass Spectrometry Methods by Paula Meleady - 9780323903950

Proteomics Mass Spectrometry MethodsSample Preparation, Protein Digestion, and Research Protocols\nAuthor(s): Paula Meleady\nFormat: Paperback\nPublisher: Elsevier Science & Technology, United Kingdom\nImprint: Academic Press Inc\nISBN-13: 9780323903950, 978-0323903950\nSynopsis\nProteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiqui.