RNA Scaffolds - 9781071615010

RNA ScaffoldsMethods and Protocols\nAuthor(s): Luc Ponchon\nFormat: Paperback\nPublisher: Springer-Verlag New York Inc., United States\nImprint: Humana\nISBN-13: 9781071615010, 978-1071615010\nSynopsis\nThis second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible labor.